Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film

Abstract： Horseradish peroxidase monolayer was assembled on the surface of PET-CO2￣ substrate. The reaction kinetics of HRP/PET film and H2O2 in micro reactor was studied using improved spectrophotometer. The relative activity of self-assembly HRP/PET film still remains above 80% after storing for 150 days at 4℃. When applied to determination of H2O2 in sample, the recoveries of H2O2 are 96.5%~101.1%. 作 者： Xiu Ming JIANG    Zhi Chun CHEN    Shao Ming YANG    Han Feng LIN    Xian Fu LIN   作者單位： Xiu Ming JIANG(Chemistry Department of Zhejiang University, Hangzhou ,310027;Chemistry Department of Zhengzhou Institute of Technology, Zhengzhou, 450052)

Zhi Chun CHEN,Shao Ming YANG,Han Feng LIN,Xian Fu LIN(Chemistry Department of Zhejiang University, Hangzhou ,310027) 

期 刊： 中國化學(xué)快報(英文版)  ISTICSCI   Journal： CHINESE CHEMICAL LETTERS  年，卷(期)： 2004, 15(5)  分類號：   Keywords： Electrostatic self-assembly film    horseradish peroxidase (HRP)    spectrophotometry    atomic force microscope (AFM)